Styrene-maleic anhydride (SMA) copolymers are used as a detergent-free approach for membrane protein solubilization, isolation and subsequent characterization in downstream methods.
SMA copolymers have proven their value in the solubilization, purification, and characterization of a variety of membrane proteins, as described in over 200 scientific articles including comprehensive review articles. An up-to-date literature overview is being kept tracked of on the page of our partner www.SMALP.net.
By clicking on this link below you will find the elaborated scientific research in the form of a PhD thesis of SMA copolymers in the solubilization of model lipid membranes and study of photosynthetic membrane proteins (2016). The author, Stefan Scheidelaar PhD, is currently the SMALP® expert in our Polyscience® team.
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In a single detergent-free step, membrane proteins are isolated with co-purified native lipids forming Styrene Maleic Acid Lipid Particles (SMALP), sometimes referred to as native nanodiscs or Lipodisqs™.
Compared to traditionally used detergents, SMA copolymers offer several advantages including the following:
MA copolymers for membrane protein isolation are available under the trade name SMALP® supplied by PolyScience®. Currently the following grades are available:
These are all aqueous and ready-to-use polymer solutions. Simply add SMALP® to your membrane suspension and purify your target membrane protein using widely used downstream techniques including, SEC, HPLC and Affinity-tag chromatography (including His, FLAG, Strep-tag, etc). SMALP® is compatible with many functional & ligand screening assays and biophysical methods, including, Crystallography, EM, DLS, CD, NMR, ESR, SPR, Mass Spectrometry, etc.